Molecular medicine is a field of medical science that combines biology, chemistry, physics, bioinformatics and engineering technology to explain about molecular structures and mechanism. Molecular methods are widely used for diagnosis, prevention, and control of a various infectious disease. Advancement in this field will help to treat the infectious diseases more accurately and effectively and to develop vaccines more safe & effective. The new advancement in this field is becoming effective to inhibit HIV, herpes virus and human papillomavirus. Monoclonal antibodies are applied in ELISA test that producing more sensitivity than traditional techniques.